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. 2017 Sep;23(9):1465–1476. doi: 10.1261/rna.060632.117

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© 2017 Cantero-Camacho et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Three-dimensional structure of subdomain SL1 determined by SAXS. (A) Secondary structure of SL1 detected by NMR spectroscopy at low and higher salt concentration (Cantero-Camacho and Gallego 2015). (B) Average ab initio envelope of SL1 calculated from the SAXS profile at low ionic strength, superposed with the best energy-minimized SL1 atomic model, selected by fitting the theoretical SAXS profiles to the experimental curve. The normalized spatial discrepancy (NSD) between scattering envelopes was 0.624. (C) Theoretical SAXS profile calculated from the best energy-minimized SL1 atomic model (red line), overlaid with the experimental profile obtained at low ionic concentration (black dots).