Table 2. Primers used for quantitative PCR of the β2-microglobulin gene.
| Targets | Template | Primer name | Sequence (5' - 3') | Annealing temprature (oC) | Product size |
|---|---|---|---|---|---|
| short-B2M | cDNA | B2M-F2 | CAGCAAGGACTGGTCTTTCTA | 53 | 133 |
| B2M-R | TyAswkGTCTCGATCCCA | ||||
| short-GAPDH | cDNA | GAPDH-F2 | CCTGGCCAAGGTCATCCA | 53 | 123 |
| GAPDH-R2 | CGGCCATCACGCCACAG | ||||
| gB2M -exon 1 | genomic | gB2M-i0F | GCCGAGCTCTCATTCCACC | 60 | 150 |
| gB2M-i1R | GGCTGGTAGAAGAGGGAAGAGG | ||||
| gB2M-exon 2 | genomic | gB2M-i1F | CTTTCCTTGATGTTCCTCAG | 60 | 198 |
| gB2M-e2R | TAGAAAGACCAGTCCTTGCTG | ||||
| gB2M-exon 3 | genomic | UniblockAB-F | CCTAACAAGGCGCTCATGGTC | 60 | 257 |
| gB2M-i3R | CTGCAGACAGTGAGTGCCAG | ||||
| gPATL2-exon 4 | genomic | gPATL2-i3F | CCTGGGTTTTCCTCCCTCC | 60 | 210 |
| gPATL2-i4R | GTCAAGAGCAGACTGCATGTC | ||||
| gGCG-exon 4 | genomic | gGCG-i3F | GCCACTGCTTATAGGTGAGAACC | 60 | 206 |
| gGCG-e4R | GCCTTCCTCGGCCTTTCA |
Note: Lower case letters for nucleotide sequences indicate the degenerated nucleotides.