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. 2017 Aug 16;12(8):e0182981. doi: 10.1371/journal.pone.0182981

Fig 6. 18β-GA-d increased cell proliferation in HaCaT keratinocytes.

Fig 6

(A) HaCaT keratinocytes were treated with 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM) for 24 h. The relative cell number was analyzed by MTT assay. (B) Similar to (A), relative cell number was determined using the trypan blue exclusion assay. (C) HaCaT keratinocytes were treated with 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM), and an in vitro scratch wound healing assay was performed. Cell migration was observed at different times (0 and 24 h). Scale bar = 200 μm. *p < 0.05 (mean ± S.E.M. n = 3).