Fig 7. 18β-GA-d induced AQP-3 expression in HaCaT keratinocytes.
(A) HaCaT keratinocytes were treated with 0.1% THF or 18β-GA-d (30 μM) for different times (6, 12, 24, and 48 h). AQP-3 protein expression was analyzed by western blotting. (B) HaCaT keratinocytes were treated with 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM) for 48 h. AQP-3 protein expression was analyzed by western blot assay. The quantification data for ratios of AQP-3/α-tubulin in 18β-GA-d-treated group, relative to AQP-3/α-tubulin in THF-treated group are shown in the lower panel. *p < 0.05 (mean ± S.E.M. n = 3).