Table 2. Design matrix of outcomes from a Plackett-Burman experimental design for the screening of significant nutrient factors.
N1 to N11 represent the nutrient factors in the following order with the low and high uncoded values in brackets: casein (1&7 g/L), peptone (1&7 g/L), lactose (1&5 g/L), sucrose (1&5 g/L), fructose (1&5 g/L), maltose (1&5 g/L), nano3 (0.5&5 g/L), glucose (1&5 g/L), L-glutamine (2&6 g/L), yeast extract (0.5&2g/L) and beef extract (0.5&2 g/L). E.A = Enzyme activity IU/mL, P.E.A = Predicted Enzyme Activity IU/mL.
| Sl. No | N1 | N2 | N3 | N4 | N5 | N6 | N7 | N8 | N9 | N10 | N11 | E.A | P.E.A | Error |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | -1 | 1 | -1 | -1 | -1 | 1 | 1 | 1 | -1 | 1 | 303.84(±4.46) | 302.11 | 1.7317 |
| 2 | 1 | 1 | -1 | 1 | -1 | -1 | -1 | 1 | 1 | 1 | -1 | 331.59(±2.46 | 330.2 | 1.3917 |
| 3 | -1 | 1 | 1 | -1 | 1 | -1 | -1 | -1 | 1 | 1 | 1 | 296.23(±8.85) | 297.96 | 1.7317 |
| 4 | 1 | -1 | 1 | 1 | -1 | 1 | -1 | -1 | -1 | 1 | 1 | 71.73(±3.34) | 70.34 | 1.3917 |
| 5 | 1 | 1 | -1 | 1 | 1 | -1 | 1 | -1 | -1 | -1 | 1 | 84.71(±1.46) | 82.98 | 1.7317 |
| 6 | 1 | 1 | 1 | -1 | 1 | 1 | -1 | 1 | -1 | -1 | -1 | 194.33(±2.77) | 192.6 | 1.7317 |
| 7 | -1 | 1 | 1 | 1 | -1 | 1 | 1 | -1 | 1 | -1 | -1 | 329.14(±5.04) | 330.53 | 1.3917 |
| 8 | -1 | -1 | 1 | 1 | 1 | -1 | 1 | 1 | -1 | 1 | -1 | 86.89(±2.75) | 88.62 | 1.7317 |
| 9 | -1 | -1 | -1 | 1 | 1 | 1 | -1 | 1 | 1 | -1 | 1 | 164.18(±4.97) | 165.57 | 1.3917 |
| 10 | 1 | -1 | -1 | -1 | 1 | 1 | 1 | -1 | 1 | 1 | -1 | 139.64(±6.95 | 138.25 | 1.3917 |
| 11 | -1 | 1 | -1 | -1 | -1 | 1 | 1 | 1 | -1 | 1 | 1 | 189.37(±1.89) | 191.1 | 1.7317 |
| 12 | -1 | -1 | -1 | -1 | -1 | -1 | -1 | -1 | -1 | -1 | -1 | 41.29(±3.54) | 42.68 | 1.3917 |