Table 4. Full factorial central composite design matrix showing predicted and real values in the optimization of L-glutaminase yield with the screened nutrient factors.
E.A = Enzyme activity IU/mL, P.E.A = Predicted Enzyme Activity IU/mL. Corresponding five levels of the actual values were L-glutamine (1, 3.5, 6, 8.5 & 11 g/L), Lactose (0.5, 2, 3.5, 5& 6.5 g/L), Glucose (0.5, 2, 3.5, 5 & 6.5 g/L), Peptone (1, 3, 5, 7 & 9 g/L) and Fructose (1, 2.5, 4, 5.5 & 7 g/L).
| L-Glutamine | Lactose | Glucose | Peptone | Fructose | P. E. A | E. A | Error |
|---|---|---|---|---|---|---|---|
| -1 | -1 | -1 | -1 | -1 | 454.55 | 453.11 | -1.4447 |
| 1 | -1 | -1 | -1 | -1 | 474.31 | 473.14 | -1.1683 |
| -1 | 1 | -1 | -1 | -1 | 461.73 | 468.55 | 6.8211 |
| 1 | 1 | -1 | -1 | -1 | 487.23 | 491.31 | 4.0800 |
| -1 | -1 | 1 | -1 | -1 | 452.38 | 457.1 | 4.7211 |
| 1 | -1 | 1 | -1 | -1 | 482.59 | 482.92 | 0.3251 |
| -1 | 1 | 1 | -1 | -1 | 473.55 | 473.17 | -0.3755 |
| 1 | 1 | 1 | -1 | -1 | 509.51 | 504.61 | -4.8991 |
| -1 | -1 | -1 | 1 | -1 | 479.79 | 483.54 | 3.7469 |
| 1 | -1 | -1 | 1 | -1 | 491.21 | 494.5 | 3.2934 |
| -1 | 1 | -1 | 1 | -1 | 495.71 | 498.81 | 3.0977 |
| 1 | 1 | -1 | 1 | -1 | 512.87 | 505.11 | -7.7633 |
| -1 | -1 | 1 | 1 | -1 | 489.2 | 491.31 | 2.1078 |
| 1 | -1 | 1 | 1 | -1 | 511.08 | 511.74 | 0.6617 |
| -1 | 1 | 1 | 1 | -1 | 519.11 | 515.67 | -3.4439 |
| 1 | 1 | 1 | 1 | -1 | 546.74 | 551.23 | 4.4925 |
| -1 | -1 | -1 | -1 | 1 | 464.21 | 470.1 | 5.8943 |
| 1 | -1 | -1 | -1 | 1 | 475.1 | 475.1 | -0.0018 |
| -1 | 1 | -1 | -1 | 1 | 454.99 | 449.51 | -5.4773 |
| 1 | 1 | -1 | -1 | 1 | 471.63 | 468.29 | -3.3409 |
| -1 | -1 | 1 | -1 | 1 | 455.29 | 450.13 | -5.1574 |
| 1 | -1 | 1 | -1 | 1 | 476.65 | 480.42 | 3.7741 |
| -1 | 1 | 1 | -1 | 1 | 460.06 | 465.01 | 4.9485 |
| 1 | 1 | 1 | -1 | 1 | 487.17 | 482.12 | -5.0476 |
| -1 | -1 | -1 | 1 | 1 | 476.76 | 481.39 | 4.6309 |
| 1 | -1 | -1 | 1 | 1 | 479.32 | 473.91 | -5.4051 |
| -1 | 1 | -1 | 1 | 1 | 476.29 | 471.55 | -4.7358 |
| 1 | 1 | -1 | 1 | 1 | 484.59 | 491.22 | 6.6307 |
| -1 | -1 | 1 | 1 | 1 | 479.43 | 479.04 | -0.3857 |
| 1 | -1 | 1 | 1 | 1 | 492.44 | 488.87 | -3.5743 |
| -1 | 1 | 1 | 1 | 1 | 492.94 | 495.99 | 3.0451 |
| 1 | 1 | 1 | 1 | 1 | 511.71 | 505.23 | -6.4809 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 480.54 | -1.2399 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 489.33 | 7.5501 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 477.27 | -4.5099 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 479.39 | -2.3899 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 489.38 | 7.6001 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 475.16 | -6.6199 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 472.28 | -9.4999 |
| 0 | 0 | 0 | 0 | 0 | 481.78 | 487.32 | 5.5401 |
| -2.366 | 0 | 0 | 0 | 0 | 439.28 | 432.11 | -7.1694 |
| 2.366 | 0 | 0 | 0 | 0 | 484.85 | 491.38 | 6.5318 |
| 0 | -2.366 | 0 | 0 | 0 | 475.81 | 471.17 | -4.6440 |
| 0 | 2.366 | 0 | 0 | 0 | 507.09 | 511.1 | 4.0064 |
| 0 | 0 | -2.366 | 0 | 0 | 487.53 | 484.22 | -3.3083 |
| 0 | 0 | 2.366 | 0 | 0 | 517.04 | 519.71 | 2.6708 |
| 0 | 0 | 0 | -2.366 | 0 | 465.81 | 464.7 | -1.1079 |
| 0 | 0 | 0 | 2.366 | 0 | 524.7 | 525.17 | 0.4703 |
| 0 | 0 | 0 | 0 | -2.366 | 485.81 | 480.22 | -5.5884 |
| 0 | 0 | 0 | 0 | 2.366 | 455.79 | 460.74 | 4.9508 |
| 0 | 0 | 0 | 0 | 0 | 482.16 | 481.22 | -0.9405 |
| 0 | 0 | 0 | 0 | 0 | 482.16 | 483.35 | 1.1895 |
| 0 | 0 | 0 | 0 | 0 | 482.16 | 491.39 | 9.2295 |
| 0 | 0 | 0 | 0 | 0 | 482.16 | 475.87 | -6.2905 |
The corresponding five levels of the actual values were L-glutamine (1, 3.5, 6, 8.5 & 11 g/L), Lactose (0.5, 2, 3.5, 5& 6.5 g/L), Glucose (0.5, 2, 3.5, 5 & 6.5 g/L), Peptone (1, 3, 5, 7 & 9 g/L) and Fructose (1, 2.5, 4, 5.5 & 7 g/L) (E.A = Enzyme activity IU/mL, P.E.A = Predicted Enzyme Activity IU/mL).