Table 1. Expression of 10 TLRs, β-defensin and peptidoglycan recognition protein 1(PGLYRP1) in rumen of kids at 3, 5, 7, 14, 21 and 28 days old fed on two feeding systems (Natural and Artificial).

ΔC_t = C_{t (TLRs)} − C_{t (βactin)}, lower ΔC_t represent higher mRNA abundance level and vice versa.

Feeding	Age	TLR1	TLR2	TLR3	TLR4	TLR5	TLR6	TLR7	TLR8	TLR9	TLR10	β-defensine	PGLYRP1
Natural	3	11.52	23.92	12.01	9.64	22.34	9.95	10.81	15.04	11.77	17.00	16.78	10.81
	5	11.51	24.19	12.79	10.53	24.03	9.58	11.18	14.88	12.21	16.60	15.58	10.74
	7	10.11	18.90	10.89	9.32	23.97	8.76	9.12	11.73	10.31	14.31	15.80	10.58
	14	11.40	20.36	10.85	10.58	25.22	8.61	12.42	12.76	13.17	15.04	17.63	15.44
	21	11.31	ND	11.95	10.71	23.69	10.40	12.64	13.05	11.33	15.21	15.11	15.48
	28	11.87	19.62	10.66	10.33	22.15	9.07	9.71	13.10	10.47	15.71	15.38	14.53
Artificial	3	10.48	24.79	10.53	9.99	20.78	8.59	10.95	14.45	11.45	16.47	15.79	10.70
	5	10.58	24.74	10.94	10.15	20.86	8.88	10.98	14.07	12.17	16.78	15.21	10.44
	7	11.07	19.97	11.44	10.18	23.72	8.86	10.54	13.41	11.51	15.89	15.33	11.31
	14	11.62	18.99	11.86	10.68	22.63	9.01	10.31	13.50	11.18	15.82	15.87	11.81
	21	11.28	ND	10.72	10.15	23.04	9.23	10.57	13.21	11.08	16.15	15.00	14.49
	28	11.64	20.19	10.68	10.41	23.23	9.43	10.60	13.89	11.37	15.68	15.10	14.89
	SED	0.638	1.121	0.916	1.092	1.384	0.612	1.814	1.122	1.487	0.831	1.249	1.721
P-value	Feeding	0.47	0.49	0.13	0.88	0.0235	0.078	0.61	0.41	0.87	0.10	0.13	0.28
	Age	0.081	<0.0001	0.39	0.51	0.06	0.15	0.54	0.0201	0.52	0.0108	0.22	<0.0001
	Interaction	0.18	0.62	0.099	0.85	0.16	0.065	0.49	0.43	0.56	0.29	0.89	0.39

SED: standard error of difference.

ND: Not determined