Fig. 3.

The morning activity peak is broader in Sik3 RNAi flies. (A) Average locomotor activity of 23–32 flies under LD (Upper) or during the transition from LD to DD (Lower). White bars represent day, gray bars represent subjective day, and black bars represent night or subjective night. ZT or CT is indicated on the x axes. The morning activity peak appears to be broader in flies expressing Sik3 dsRNAs in sLNvs. (B) Quantification of the width of the morning activity peak at 80% of peak activity under LD (Left, six independent experiments) and during the transition from LD to DD (Right, four independent experiments) confirms that morning activity is spread over a longer period. **P < 0.01, ***P < 0.001, ****P < 0.001 (one-way ANOVA analysis followed by Tukey’s multiple comparisons test; ANOVA P < 0.0001 for LD and P < 0.001 for LD to DD).