Fig. S6.

Sik3 knockdown in sLNvs changes the amplitude and phase of PER cycling in DN1ps. Shown is the relative intensity of nuclear PER expression in sLNvs, LNds, and DN1ps at different circadian times of flies kept in DD. (A) Measurements as in Fig. 2B of another biological sample (2 ≤ n ≤ 6). (B–D) Measurements from third subjective night to the fourth subjective day of single (5 ≤ n ≤ 6) (B) or female-paired (n = 6) (C) Sik3^RNAi males and control males and singly kept Sik3^RNAi males and controls in which SIK3 was knocked down with another driver (Pdf-GAL4; 5 ≤ n ≤ 10) (D). In all cases, PER cycling of experimental flies is out of phase or there is no clear peak in DN1 neurons as compared with LNds or sLNvs. (E and F) Dcr2 expression does not cause low amplitude or a phase shift in PER cycling in DN1 neurons. Measurements were from the third subjective day to the third subjective night (E: 6 ≤ n ≤ 8; F: 3 ≤ n ≤ 6). Error bars represent SEM. **P < 0.01 and ****P < 0.0001 (one-way ANOVA followed by Tukey’s multiple comparison test); ns, not significant. This figure is related to Fig. 2.