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. 2017 Jul 25;114(32):E6669–E6677. doi: 10.1073/pnas.1620483114

Fig. S8.

Fig. S8.

Nucleocytoplasmic cycling of HDAC4 in CRY+ LNds. (A) The graph displays lLNvs with nuclear HDAC4 at different times of day. There is no significant difference in values (ANOVA followed by Tukey’s multiple comparisons, P < 0.05). (B and C) HDAC4 nucleocytoplasmic localization cycles in CRY+ and PDFR+ LNds (cry-GAL4/UAS-HDAC4-FLAG in B and Mai179/Pdf-GAL80/UAS-HDAC4-FLAG in C). Note that Mai179 labels only CRY+ and PDFR+ cells in LNds. Arrows and arrowheads in B indicate LNds with nuclear-localized HDAC4 and putative CRY− and PDFR− LNds, respectively. HDAC4, CLK, and PER were visualized by anti-FLAG, anti-CLK, and anti-PER antibodies, respectively. (Scale bars: 5 μm.) (D–F) HDAC4 localization in LNds of flies overexpressing HDAC43A (cry-GAL4/UAS-HDAC43A-FLAG) (D), overexpressing ΔCYC (cry-GAL4/UAS-ΔCYC/UAS-HDAC4-FLAG) (E), or reducing Sik3 by RNAi (cry-GAL4/UAS-Dcr2/UAS-Sik3RNAi/UAS-HDAC4-FLAG) (F). (Scale bars: 5 μm.) (B′ –F′) Graphs display LNds with nuclear HDAC4 (B′, C′, E,′ and F′) or HDAC43A (D′) in brains shown in B–F at different times of day. Letters in graphs indicate significant differences in values (P < 0.05, ANOVA followed by Tukey’s multiple comparisons). This figure is related to Figs. 4 and 5.