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. 2017 Jul 24;114(32):8620–8625. doi: 10.1073/pnas.1700917114

Fig. 1.

Fig. 1.

Car6-b is preferentially expressed in macrophages. (A) The structure of the A and B type of Car6 in genome. SP, signal peptide; UTR, untranslated region. (B) RT-PCR analysis of Car6-a and Car6-b in immune cells; Actb serves as a loading control. Car6-a expressed in the salivary gland serves as a positive control. (C) qPCR analysis of Car6-b in mouse immune cells; results were normalized to those of Actb. (D and E) Car6-b expression in PMs after stimulation by LM (D) or LPS (E) for indicated times. Data were shown as mean ± SD (n = 3). *P < 0.05, **P < 0.01 ***P < 0.001. (F and G) Immunofluorescence assay of the location of CA-VI in 293T cells (F) and PMs (G) stimulated with LM or LPS for 2 h or left unstimulated (Med). (Scale bar for F and G, 10 µm.)