Table 1.
Novel genetic interactions with ubc-18 identified from the RNAi screen
| Gene | Synthetic phenotype | Process | Function/homology |
| ubc-3 | Lethal | Ubiquitination | E2 specific to SCF type E3; regulates G1 and G2 in cell cycle |
| skr-2 | Lethal | Ubiquitination | Skp1-related component of SCF type E3 ubiquitin ligase complex |
| skr-1 | Lethal | Ubiquitination | Skp1-related component of SCF type E3 ubiquitin ligase complex |
| egl-27 | Lethal | Chromatin regulation | MTA (human metastasis) homolog, nucleosome remodeling |
| lin-54 | Lethal | Nucleic acid regulation | DNA binding |
| Y65B4A.1 | Lethal | Nucleic acid regulation | Nucleotide binding |
| smu-2 | Lethal | Nucleic acid regulation | RNA splicing |
| emb-4 | Lethal | Nucleic acid regulation | Ortholog to human spliceosome |
| dpy-10 | Lethal | Body morphology and movement | Cuticle collagen protein |
| prx-13 | Lethal | Proteolysis | Human PEX13 homolog (endopeptidase); human Zellweger syndrome |
| ptc-1 | Lethal | Cytokinesis | Sugar modification; eggshell development |
| B0035.1 | Lethal | Unknown | ZNF207/BUGZ ortholog |
| T09A5.9 | Lethal | Unknown | PPP1R7 homolog (protein phosphatase) |
| fbf-2 | Sterile | Nucleic acid regulation | RNA binding |
| mrpl-10 | Sterile | Mitochondrial translation | Mitochondrial ribosomal protein, large subunit |
| trr-1 | Sterile | Chromatin regulation | Homolog of protein kinase in chromatin remodeling complex; synMuv |
Validated novel hits from RNAi screen using two ubc-18 loss-of-function alleles: ubc-18(ku354) and ubc-18(tm5426). Our screen also identified lin-35, previously shown to be synthetically lethal with ubc-18 (8). Process and function/homology are from Wormbase build WS254 (www.wormbase.org).