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. 2017 Jul 10;114(32):E6632–E6641. doi: 10.1073/pnas.1705508114

Fig. 3.

Fig. 3.

Quantitation of DNA release events by NTHI. In rich medium (sBHI), we observed an average of 17 events per 1,000 parent cells after 2 h (solid red bar). There were no detectable events for either the ΔcomE or ΔtraCG mutant at this time point. Complementation of the mutations restored the average number of release events similar to that of the parent (compare solid red bar with green, blue, and yellow bars; no statistical differences). Induction of competence by incubation of NTHI in sBHI + cAMP (red striped bar) increased the number of DNA release events. This increase was statistically significant when NTHI was incubated in M-IV medium (red stippled bar). The number of DNA release events was calculated per 1,000 cells. Data are shown as the mean ± SEM from five separate sets of 1,000 cells (5,000 total cells evaluated). Statistical significance was calculated using one-way ANOVA with significance shown at P ≤ 0.001.