Fig. S2.

Transformation frequency in M-IV nutrient-limited medium. Box and whiskers plot depicting mean transformation frequencies for the parent, ΔcomE mutant, ΔtraCG mutant, and complemented ΔtraCG mutant. No statistically significant differences in transformation frequency were noted among the parental isolate, ΔtraCG mutant, or complemented ΔtraCG mutant, whereas no transformants were observed for the ΔcomE mutant. These data suggested that the inner-membrane complex formed by TraCG did not play a role in the uptake of DNA from the environment. Mean efficiency for each strain was calculated from four replicate experiments. Whiskers indicate minimum to maximum.