Table S2.
Primer sequences
| Allele | Round | Forward primer 5′ → 3′* | Reverse primer 5′ → 3′ |
| VB1-8 productive | First | CATGGGATGGAGCTGTATCAT | AAGGCTCTGAAATCGATACCGT |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXCTTTCTCTCCACAGGTGTCC | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTAAGAGAGAGGTTGTAAGGACTCACC | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTAAGAGAGAGGTTGTAAGGACTCACC | |
| VH1-2+CDR3VB1-8 passenger | First | ATGGGATGGAGCTGACTCAT | TCTGTCGACGATATCGGATC |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXATAGGCGCTATGGCGCGA | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTAAGAGAGAGGTTGTAAGGACTCACC | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTAAGAGAGAGGTTGTAAGGACTCACC | |
| VH1-2+CDR3VRC-PG04, int6.1%, int6.8% passengers | First | ATGGGATGGAGCTGACTCAT | CCGCTCTAGCCTCGAGTACGTAAG |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXCTTTCTCTCCACAGGTGTCC | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCTGTCGACGATATCGGATC | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCTGTCGACGATATCGGATC | |
| int16.6%, VRC-PG04 passengers | First | ATGGGATGGAGCTGACTCAT | CCGCTCTAGCCTCGAGTACGTAAG |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXCTTTCTCTCCACAGGTGTCC | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCTGTCGACGATATCGGATC | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCTGTCGACGATATCGGATC | |
| VH1-2–rearranging mice (22) (homozygous IGHV1-2*02/ ΔIGCR1) | First | TGGACCTGGAGGATCCTCTT | GGCTCTGAGATCCCTAGACAG |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXTTCTCTCCACAGGAGCCCAC | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCTGTCGACGATATCGGATC | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC | |
| VH1-2–rearranging mice (22) (IGHV1-2*02/ ΔIGCR1 and human JH2 knock-in on one IgH allele, JH deletion on other IgH allele) | First | TGGACCTGGAGGATCCTCTT3' | GGCTCTGAGATCCCTAGACAG |
| Second | TACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXTTCTCTCCACAGGAGCCCAC | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTGGACAGAGAAGACTGGGAGG | |
| Third | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTGGACAGAGAAGACTGGGAGG |
*
XXXXXX are nucleotides that are unique to each library (barcodes).