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. 2017 Jul 17;114(32):E6585–E6594. doi: 10.1073/pnas.1710751114

Fig. S2.

Fig. S2.

3D-SIM of recovered actin and lytic granules at the synapse. (A) Representative 3D-SIM projection of recovered cortical actin in a phalloidin-stained OT-I CTL expressing LAMP1-EGFP fixed 10 min after stimulation on an anti-CD3–coated glass surface. (B) 3D SIM image rotated 90° in the z plane to visualize actin (Top, grayscale), LAMP1 (Middle, grayscale), or the merged image (Bottom) showing LAMP1+ granules on the cytosolic side of recovered cortical actin. Granules are highlighted by a yellow dotted line in the LAMP1 panel. (Scale bars: 5 μm.) Dashed white lines numbered 1, 2, and 3 in the merged image of B demarcate the areas of the line scan graphs shown below (dashed line 1 demarcates linescan 1; dashed line 2 demarcates linescan 2; dashed line 3 demarcates linescan 3) showing phalloidin (red) and LAMP1 (green) pixel intensities across the region of interest. Data are representative of n = 5 cells from two independent experiments. AU, arbitrary units.