Fig. S3.

CTL function in U-73122–treated CTLs is rescued by PMA/ionomycin. Intracellular cytokine production is inhibited in U-73122–treated CTLs but is rescued by PMA plus ionomycin treatment in the presence of the inhibitor. In vitro-activated OT1 CTLs were treated with either U-73122 or U-73433 control (both at 1 μM final concentration) and were stimulated for 6 h with 5 μg/mL plate-bound anti-CD3 or were left unstimulated as a control ± PMA (20 ng/mL final concentration) and ionomycin (1 μg/mL final concentration). Data shown are representative of two independent experiments.