Fig. S4.

5-Ptase recruited by a rapamycin-inducible dimer system reduces levels of PIP₂ in the plasma membrane. (A) Schematic of the inducible dimer system for the manipulation of plasma membrane-localized PIP₂. A cytosolic 5-ptase linked to mRFP is recruited to the plasma membrane via rapamycin-dependent heterodimerization of FKBP12 with membrane-bound FRB. (B) Representative TIRF images of COS7 cells expressing mRFP-FKBP12-5-ptase (i and ii), Tubby-EGFP (iii and iv), and tagBFP2-FRB-PM (not pictured) before treatment with rapamycin (i, iii, and v) and after treatment (ii, iv, and vi). Merged before and after images are shown in v and vi, respectively (Scale bar: 10 μm.)