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. 2017 Aug 16;12(8):e0183026. doi: 10.1371/journal.pone.0183026

Fig 4. DCS (20 mg/kg/day IP) partially restores proBDNF (pBDNF) in the brainstem and striatum of Mecp2tm1.1Jae/y mice.

Fig 4

A. The brainstem and striatum but not hippocampus (HPC) had lower levels of total BDNF (tBDNF) protein. DCS had no effect on BDNF protein levels in any brain region in WT mice. DCS increased BDNF in the brainstem and striatum of Mecp2tm1.1Jae/y mice. DCS had no effect on BDNF levels in the hippocampus. B. The HPC, brainstem and striatum had lower levels of mature BDNF (mBDNF) and DCS had no effect on protein levels in any of the brain regions. C. The brainstem and striatum but not HPC had lower levels of proBDNF (pBDNF) protein. DCS had no effect on pBDNF in any brain region in WT mice, however it increased pBDNF levels in the brainstem and striatum of Mecp2tm1.1Jae/y mice. WT VEH n = 8, WT DCS n = 7–8, Mecp2tm1.1Jae/y VEH n = 8, Mecp2tm1.1Jae/y DCS n = 6. *p < .05, ***p < .001, ****p < .0001. D. Representative western blot images showing mBDNF, pBDNF and GAPDH in HPC, brainstem and striatum of all groups.