Table 1. Transformation efficiency and frequency of plasmids in electroporation of A. caldus MTH-04.
Transformation efficiencya (×104 CFU/μg DNA) |
Transformation frequencyb (×10−6 CFU/cells added) |
|
---|---|---|
pJRD215 | 1.09±0.11 | 2.55±0.25 |
pSDU1 | 30.43±0.88 | 71.00±2.05 |
pSDU1Δmob | 38.67±0.79 | 90.22±1.85 |
pSDU1ΔmobΔorfEF | 0.12±0.01 | 0.28±0.03 |
a Mean±Standard deviation.
b Mean±Standard deviation.