Table 6. Primers used in this work.
Name | Sequence (5’ to 3’)a |
---|---|
Cm S1sen | GCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGGAGAAAAAAATCACTGG |
CmS2 sen | CTGAGAATTCATGCATTCATGTTTGACAGCTTATCATCCATAAGGTTTAATGCGGTAGTTTATCACAG |
Cm ant | CTTCGTGCACATTCATCCGCTTATTATCACTT |
P215 sen | CTTCGTGCACTCCTTGCAATACTGTGTT |
P215 ant | CTTCATGCATCCCAAGCTTAGAGCATACATCTGGAAGCA |
MCS sen | CTAGTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCATGCATTCATGTTTGACAGCT |
MCS ant | CTAGTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGAGCATACATCTGGAAGCA |
RepB S1 sen | TGGAGGCACAGCATTGAGCCGAAAAGCAAAAGCAACAGCGAGGCAGCATGAAGAACGACAGGACT |
RepB S2 sen | TCCCCTTAACCATCTTGACACCCCATTGTTAATGTGCTGTCTCGTAGGCTATCATGGAGGCACAGCATTG |
Remove mob ant | GCTGAATGATCGACCGAGAC |
orfEF sen | ATGGCTACCCATAAGCCTATCAAT |
orfEF ant | AAAACCCCCTTCTGTGCGTGAGT |
RepA sen | CGGGTGCTCTATCGTGTTCCTG |
RepA ant | GCGGATGTTATCGACCAGTACC |
tetH sen | ACGGCGTTAAGGAAGCACTG |
tetH ant | GTCGTCACTTTCGGCATAGA |
tetH Xba sen | CTAGTCTAGAACGGCGTTAAGGAAGCACTG |
tetH Hind ant | TCCCAAGCTTGTCGTCACTTTCGGCATAGA |
a Artificial restriction sites are italic and underlined.