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. 2017 Aug 16;12(8):e0183307. doi: 10.1371/journal.pone.0183307

Table 6. Primers used in this work.

Name Sequence (5’ to 3’)a
Cm S1sen GCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGGAGAAAAAAATCACTGG
CmS2 sen CTGAGAATTCATGCATTCATGTTTGACAGCTTATCATCCATAAGGTTTAATGCGGTAGTTTATCACAG
Cm ant CTTCGTGCACATTCATCCGCTTATTATCACTT
P215 sen CTTCGTGCACTCCTTGCAATACTGTGTT
P215 ant CTTCATGCATCCCAAGCTTAGAGCATACATCTGGAAGCA
MCS sen CTAGTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCATGCATTCATGTTTGACAGCT
MCS ant CTAGTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGAGCATACATCTGGAAGCA
RepB S1 sen TGGAGGCACAGCATTGAGCCGAAAAGCAAAAGCAACAGCGAGGCAGCATGAAGAACGACAGGACT
RepB S2 sen TCCCCTTAACCATCTTGACACCCCATTGTTAATGTGCTGTCTCGTAGGCTATCATGGAGGCACAGCATTG
Remove mob ant GCTGAATGATCGACCGAGAC
orfEF sen ATGGCTACCCATAAGCCTATCAAT
orfEF ant AAAACCCCCTTCTGTGCGTGAGT
RepA sen CGGGTGCTCTATCGTGTTCCTG
RepA ant GCGGATGTTATCGACCAGTACC
tetH sen ACGGCGTTAAGGAAGCACTG
tetH ant GTCGTCACTTTCGGCATAGA
tetH Xba sen CTAGTCTAGAACGGCGTTAAGGAAGCACTG
tetH Hind ant TCCCAAGCTTGTCGTCACTTTCGGCATAGA

a Artificial restriction sites are italic and underlined.