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Effective protein–lipid complex formation requires the presence of high amounts of detergent micelles. (A) Complexes between MaSP1-NT and POPG could be observed only in the presence of C8E4 above the cmc. (B) Detergents with lower cmc did not promote binding of POPG to MaSp1-NT. However, when the concentration of C10E6 was increased to that of C8E4, moderate lipid binding could be observed.
