. Author manuscript; available in PMC: 2018 Aug 12.

Published in final edited form as: Biochem Biophys Res Commun. 2017 May 30;490(1):8–16. doi: 10.1016/j.bbrc.2017.05.153

Table 1. Primers of precision tagging for THSD1.

All the primers for THSD1 precision tagging were listed. Type IIS restriction enzyme recognition sequences are marked by red letters. Custom sticky ends belonging to the THSD1-specific sequences are highlighted in green letters. Tag-specific sequence were marked by square bracket. Note that different tags may require different Type IIS restriction enzymes (BsaI, BbsI and BsmBI) to avoid internal cleavage in the tag.

Primer name	Sequence	Type IIS enzyme
HA-sense		N/A
HA-antisense		N/A
Myc-sense		N/A
Myc-antisense		N/A
FLAG-sense		N/A
FLAG-antisense		N/A
V5-sense		N/A
V5-antisense		N/A
AviTag-sense		N/A
AviTag-antisense		N/A
Strep-Tag II-sense		N/A
Strep-Tag II-antisense		N/A
EGFP-F		BbsI
EGFP-R		BbsI
mCherry-F		BsaI
mCherry-R		BsaI
Dendra2-F		BsmBI
Dendra2-R		BsmBI
SNAPf-F		BbsI
SNAPf-R		BbsI
miniSOG-F		BbsI
miniSOG-R		BbsI