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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Cancer Res. 2010 Dec 15;70(24):10080–10089. doi: 10.1158/0008-5472.CAN-10-0324

Figure 5. Tumor Development and Metastasis in the Snail transgenic mouse.

Figure 5

7–8 week old mice were treated with DMBA+TPA unless otherwise noted. (A) Histological staining with hematoxylin and eosin (H&E) of wild type (WT) and Snail transgenic (Tg) ± DMBA and TPA. (B) Oil Red O staining to detect sebaceous glands in WT(left panel) and transgenic (middle panel) skin. Right panel is a transgenic skin section stained with H&E – arrowheads point to blood vessel. (C) H&E of Snail transgenic skin demonstrating an epidermal ulcer (left panel), invasion into adipose tissue, and stromal tissue (asterisk). (D) Upper panels: H&E of lymph nodes from wild type (left panel) and transgenic (middle panel, 10X; right panel, 40X) mice treated with DMBA and TPA. Lower panels: immunofluorescence of keratin 5 (red) in lymph nodes. Bars, 30μm.