Crocus sativus extract (50 mg/kg/day, one-month treatment) and to a lesser extent crocin (10 mg/kg/day, one month treatment) consumption significantly reduced Aβ burden in the hippocampus of 5XFAD mice. (A) Representative hippocampus sections and optical density quantification in 5XFAD mice from control, treated with Crocus sativus extract, and crocin; stained with 6E10 antibody against Aβ to detect total Aβ load (green) and Anti-Aβo antibody to detect Aβo load (green). DAPI (blue) was used to stain nuclei. (B) Soluble Aβ levels in the brains of 5XFAD mice from control, treated with Crocus sativus extract, and crocin. Data are presented as mean ± SEM of six mice in each group (*P < 0.05, **P < 0.01, ***P < 0.001 compared to control group).