Fig. 8.

In-vitro enzymatic deamination activity of the gemcitabine-lipid conjugates. Human recombinant cytidine deaminase (CDA) was used in the current study. 100 μM of free gemcitabine HCL or equivalents from gemcitabine-lipid conjugates were incubated with 0.25 μg of CDA at 37°C and the absorbance was recorded at λ = 220 nm for 60 min. the decrease in the absorbance intensity after CDA addition indicated that deamination reaction had taken place [12]. * P value < 0.05: indicates that Gem-γ-T3 was significantly less affected by deamination than the free gemcitabine HCL