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The phenazine PYO promotes Fe-depletion by CP-Ser from Tris:TSB under aerobic conditions. The medium was treated with 10.5 μM (250 μg/mL) CP-Ser and10 μM PYO at 30 ºC, 150 rpm. The metal content of Fe was analyzed by ICP-MS at t = 0, 1, 2, 4, 8, 24, and 48 h. The mean and SDM are reported (n = 3). The Fe-depletion profiles of CP-Ser in the presence and absence of BME are reproduced from Fig. 2B.
