Fig. 3.

Effects of pentosan polysulfate (PPS) sodium and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in micromass culture maintained for 12-days. Photomicrographs represent the treatment conditions as; (A) 10% DMEM -negative control (NC), (B) chondrogenic differentiation medium (CDM) -positive control (PC), (C) 1.0 µg/ml PPS, (D) 3.0 µg/ml PPS, (E) 5.0 µg/ml PPS, (F) 1.0 µg/ml PSGAG, (G) 3.0 µg/ml PSGAG and (H) 5.0 µg/ml PSGAG. The supplementation of PPS and PSGAG to CDM tended to enhance cell aggregation and PG deposition at 12-days compared to NC and PC although the difference between PPS and PSGAG was not remarkable. PG deposition was evaluated by Alcian blue stain. Results are from one representative of six independent experiments (n=6). Scale bars: 20 µm.