Figure 3.

Phylogenetic tree of FH2 domains of formins from Dictyostelium and other organisms. Amino acid sequences of the FH2 domains (core and lasso region) were aligned with ClustalX and the output file was subsequently edited manually. A bootstrapped unrooted phylogenetic tree was constructed as described in the Methods section. Dictyostelium members are indicated in red. The other organisms considered are Arabidopsis thaliana (At), Saccharomyces cerevisiae (Sc), Schizosaccharomyces pombe (Sp), Drosophila melanogaster (Dm), Caenorhabditis elegans (Ce) and Homo sapiens (Hs). Nodes supported by either >75% or >50% bootstraps have been marked with red or green circles, respectively. For simplicity, nodes outside of a cluster supported by >50% bootstraps have not been indicated. Asterisks denote novel formin subfamilies. The scale bar indicates percent substitutions.