Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 15;11:15. doi: 10.3389/fnint.2017.00015

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Härtig, Mages, Aleithe, Nitzsche, Altmann, Barthel, Krueger and Michalski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Infarcted mouse: up-regulated Coll IV and decomposed PNs in ischemic neocortex—allocated with microglia/macrophages, activated astroglia and altered parvalbumin-immunolabeling. The ischemic border zone in the somatosensory cortex (A,B) exhibits nearly complementary distribution patterns for WFA (green) and strongly up-regulated Coll IV (red) 1 day after induction of ischemia. Additionally, (A) displays in the apparently non-affected zone evenly distributed ionized calcium binding adaptor molecule 1 (Iba)-positive microglia (Cy5, color-coded in blue), whereas the infarcted zone is partly devoid of Iba-immunoreactivity (asterisks). In parallel, glial fibrillary acidic protein (GFAP)-immunolabeling (blue) in (B) reveals also fragmented astrocytes (arrows) in the damaged tissue. (C–C‴) show concomitant staining of Coll IV, WFA and parvalbumin as markers for most neocortical net-bearing neurons. Up-regulated Coll IV-immunoreactivity (C) indicates the ischemia-affected tissue, where WFA-stained nets appear decomposed (arrowheads in C′ and the merged picture C‴) or even abolished. Parvalbumin-immunolabeling (C″,C‴) visualizes shrunken neurons (exemplified by an arrow) and enhanced neuropil staining in the same area. Here, the overlay (C‴) elucidates only a few PNs around parvalbumin-containing neurons. Scale bar in A,B,C‴ (also valid for C–C″) = 75 μm.