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. 2017 Aug 16;7:8319. doi: 10.1038/s41598-017-08560-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Directly applied NTP as well as NTP-treated medium induced apoptosis more effectively in 6606PDA. 6606PDA cells (left panel) or primary, murine fibroblasts (right panel) were exposed to argon gas-treated medium (control) or NTP-treated medium. After an incubation of 24 h, cells were imaged for activated caspases 3/7 by fluorescence microscopy. Representative images of two independent experiments are shown. Scale bars represent 100 µm.