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. 2017 Aug 16;7:8319. doi: 10.1038/s41598-017-08560-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NTP-treated medium decreased number, growth, and size of pancreatic lesions in vivo. The experimental timeline (a) is given. The number of tumor nodes in the peritoneum was followed by MRI and was elevated in the control animals (I) compared to NTP-treatment group (II) at the end of treatment (d28). Also, corresponding macroscopic findings for control (III) and NTP-treated (IV) animals are shown (b). MRI-based calculation of tumor volume revealed a significantly decreased total tumor growth in the treatment group (c). At day 28, animals were sacrificed and tumor nodes were excised and weighed, showing a significantly decreased total tumor mass (d). Representative images of 13 animals are given, green arrows indicate tumor nodes (one green arrow per lesion) (b). Data are presented as mean of 8–17 animals (c,d).