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. 2017 Aug 16;7:8304. doi: 10.1038/s41598-017-08588-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of acrolein-modified SP-A in mouse lungs after 1 week of exposure to CS. Eight-week-old female C57BL/6 mice were exposed to the smoke from four unfiltered cigarettes per day for one week. (A) Upper panel: Lung tissue lysates were immunoprecipitated with anti-acrolein antibody (mAb5F6) and subjected to western blotting with anti-SP-A antibody. Lower panel: Lung tissue lysates were subjected to western blot analysis using anti-SP-A and anti-actin antibodies. Lanes 1–16 represent individual samples from n = 8 mice per group. Uncropped blots are shown in Supplementary Fig. S5. (B) Immunofluorescence staining of lung sections with anti-acrolein antibody (red), anti-SP-A antibody (green), and DAPI (blue). Scale bars: 100 μm. These experiments were performed with n = 8 mice per group, and representative results are shown.