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. 2017 Aug 16;7:8488. doi: 10.1038/s41598-017-08282-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alignment of V241I with H275Y. Using the wild-type NA(A/California/07/2009) as a reference structure, the two mutants were aligned together. Yellow: the reference NA. Magenta: NA with V241I alone. Slate: both V241I and H275Y.