Table 2.
Ca2+ cycling and spark parameters.
| Parameter name | Fresh 1Hz | Cultue 1Hz | Fresh 2Hz | Cultur 2Hz | Fresh 3Hz | Culture 3Hz |
|---|---|---|---|---|---|---|
| Time to peak [ms] | 276 ± 20 | 239 ± 7 | 208 ± 16 | 184 ± 3 | 130 ± 11 | 145 ± 2 |
| p = 0.08 | p = 0.09 | p = 0.1 | ||||
| t50 [ms] | 418 ± 26 | 412 ± 17 | 327 ± 24 | 293 ± 5 | 212 ± 7 | 227 ± 6 |
| p = 0.09 | p = 0.08 | p = 0.4 | ||||
| t90 [ms] | 653 ± 37 | 714 ± 16 | 437 ± 19 | 416 ± 5 | 277 ± 8 | 295 ± 5 |
| p = 0.16 | p = 0.1 | p = 0.2 | ||||
| Cycle length [ms] | 983 ± 33 | 999 ± 3.6 | 508 ± 1.7 | 508 ± 2 | 336 ± 8 | 342 ± 4 |
| p = 0.2 | p = 1 | p = 0.5 | ||||
| Ca2+ spark 50% duration [ms] | 28 ± 1.5 | 22 ± 1.1 | 22 ± 1.5 | 20 ± 0.8 | 20 ± 0.9 | 21 ± 0.8 |
| p = 0.1 | p = 0.24 | p = 0.18 | ||||
| Ca2+ spark amplitude [] | 1.8 ± 0.1 | 1.6 ± 0.03 | 1.3 ± 0.04 | 1.6 ± 0.04 | 1.7 ± 0.08 | 1.5 ± 0.03 |
| p = 0.09 | p = 0.09 | p = 0.09 | ||||
| Ca2+ spark length [μm] | 4 ± 0.1 | 3.9 ± 0.1 | 4.2 ± 0.18 | 4.07 ± 0.1 | 4.06 ± 0.2 | 4.6 ± 0.1 |
| p = 0.16 | p = 0.26 | p = 0.09 | ||||
| Time between Ca2+ spark and former Ca2+ peak [ms] | 562 ± 21 | 518 ± 30 | 334 ± 22 | 276 ± 12 | 169 ± 18 | 180 ± 8 |
| p = 0.2 | p = 0.1 | p = 0.1 | ||||
| Spark/μm/sec | 0.03 ± 0.01 | 0.024 ± 0.001 | 0.02 ± 0.001 | 0.02 ± 0.002 | 0.02 ± 0.001 | 0.03 ± 0.001 |
| p = 0.2 | p = 0.3 | p = 0.09 | ||||
| Number of cells | 8 | 20 | 9 | 23 | 6 | 20 |
| Number of rabbits | 6 | 3 | 6 | 3 | 6 | 3 |
| Number of Ca2+ sparks | 122 | 144 | 69 | 223 | 41 | 179 |