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. 2017 Aug 16;7:8450. doi: 10.1038/s41598-017-08493-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting and quantitation of tagged α-tubulin mEGFP and mEos 3.2 clones. (a) All three cell lines express a tagged α-tubulin at the predicted molecular weight of approximately 80 kDa. Use of an anti-GFP antibody detects these bands in eGFP expressing clones. (b) All three cell lines demonstrate a significant difference in expression between eGFP and mEos 3.2 tagged TubA1B (****p < 0.0004 in Hel 92.1.7, **p < 0.0014 in A549, and ****(p < 0.0001), with little clone to clone variation in the samples tested. Statistical testing performed through a Two-Way ANOVA with multiple comparisons (Tukey). Full length western blots and quantitation of endogenous TubA1B expression levels are supplied in Supplementary Fig. 6 and 7.