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. 2017 Aug 15;8:1545. doi: 10.3389/fmicb.2017.01545

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Copyright © 2017 Rossi, Souza-Silva, Araújo-Alves and Giambiagi-deMarval.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A multiplex PCR for screening and typing CRISPR/Cas systems in coagulase-negative staphylococci. The multiplex was designed to detect the universal cas1 gene, and to differentiate than between those belonging to types II and III CRISPR systems. The detection of the 16S rRNA is employed as an endogenous control of the reaction. S. epidermidis strains RP62A (1) and 10 L (2) were used as positive controls for the amplification of cas1 of types II and III, respectively. M: molecular weight marker.