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. 2017 Aug 15;8:1545. doi: 10.3389/fmicb.2017.01545

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Copyright © 2017 Rossi, Souza-Silva, Araújo-Alves and Giambiagi-deMarval.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of cas1 in S. haemolyticus clinical strains MD03 and MD25. The expression of type III cas1 in the clinical strains that were positive for the multiplex PCR reaction was evaluated during exponential growth (exp), stationary phase (sta) and biofilm formation (bio). M: molecular weight marker, C+: positive control (genomic DNA) and C–: negative control (RNA that was subjected to the cDNA synthesis reaction, but without the addition of the Reverse Transcriptase). The expression of the rpoB gene was used as and endogenous control.