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. 2017 Aug 15;10:256. doi: 10.3389/fnmol.2017.00256

Figure 2.

Figure 2

Syt1 stimulates cis- and trans- ternary SNARE complex formation with Munc18 and Munc13-1 in the presence of the membranes. (A) Syt1 promotes cis ternary SNARE complex formation between membrane-embedded Munc18-1/syntaxin-1 complex (t-liposomes) and cytoplasmic domain of synaptobrevin-2 in the presence of SNAP-25, the Munc13-1 C1-C2B-MUN fragment and 0.2 mM EDTA. (B) Quantification of the results in (A). (C) Syt1 stimulates trans ternary SNARE complex formation between membrane-embedded Munc18-1/syntaxin-1 complex (t-liposomes) and membrane-embedded synaptobrevin-2 (v-liposomes) in the presence of SNAP-25 Δ9, the Munc13-1 C1-C2B-MUN fragment, 0.2 mM EDTA. (D) Quantification of the results in (C). Schematic diagrams and lipid compositions were displayed on the top of each charts. Representative traces from one of three independent experiments are shown. Data in the bar charts were presented as means ± SD, n = 3, technical replicates.