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. 2017 Aug 16;7:8448. doi: 10.1038/s41598-017-09038-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic relationships based on the 3 A, 3 B, 3C, and 3D coding sequences of 379 EV-B strains. Two E-18 strains and 377 EV-B strains available in GenBank database were analysed by nucleotide sequence alignment using the neighbor joining algorithms implemented in the MEGA 6.06 program. Numbers at the nodes indicate bootstrap support for that node (percentage of 1000 bootstrap replicates). The scale bars represent the genetic distance. Only high bootstrap values (>75%) are shown. Only high bootstrap values (>75%) are shown. ▲ Strains isolated in this investigation. Red denote the other E-18 strains.