Table 3.
Primers used for complete genome amplification and sequencing.
| primer | Sequence (5′-3′) | Nucleotide position* | Orientation |
|---|---|---|---|
| 224 | GCIATGYTIGGIACICAYRT | 1977–1996 | Forward |
| 222 | CICCIGGIGGIAYRWACAT | 2969–2951 | Reverse |
| E181F | TTAAAACAGCCTGTGGGTTG | 1–20 | Forward |
| E181R | TGTGCCATGAAGGGTGTA | 2431–2414 | Reverse |
| E181r | GGAACGCGGTGACTCATC | 340–323 | Reverse |
| E181f | CTATAAGGATGCGGCATC | 839–856 | Forward |
| E182F | CATAAACGTTAGGGAGA | 2714–2730 | Forward |
| E182f | GTACTTCTCGCAGCTGGG | 3600–3617 | Forward |
| E184f | CAGAGTGATCAAGAGCA | 4263–4269 | Forward |
| E185r | CCCAACTGGGATGTACAT | 5749–5732 | Reverse |
| E186r | CCTGGGTTTTGGTGAAAG | 6520–6503 | Reverse |
| E187f | GACAAGGGAGAGTGTTT | 7018–7034 | Forward |
| E188R | ACCGAATGCGGAGAATTTAC | 7410–7391 | Reverse |
*Numbering according to the genome of E-18 strain E18-314/HB/CHN/2015.