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. 2017 Aug 16;7:8476. doi: 10.1038/s41598-017-09158-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exosome secretion, motility and invasion of cells overexpressing isoform B. (A) Fluorescence of the secreted exosomal fraction (stained with membrane dye Vybrant DiI) from PC3 cells transfected with EGFP alone (control transfection, EGFP) and EGFP fusion of myosin IC isoform B (isoB). Data were normalized to the control. N = 3 cell cultures per group. Experiment was repeated 3 times. Error bar: SE. (B) In-vitro wound closure experiment on PC3 transfected with the same constructs. Scratched and healing cell monolayers were photographed in phase contrast 24 h after scratching. Representative fields are shown. Wound area outline in cyan is overlaid by the software used for the automated measurement. (C) Quantification of the experiment in (B). Error bars: SE. N = 10 camera fields per group. Experiment reproduced 2 times. (D) Matrigel invasion experiment. Nuclei of cells that have invaded across the reconstituted extracellular matrix layer were visualized by DAPI staining. Representative fields are shown. (E) Quantification of the experiment in (D). Error bars: SE. N = 18 camera fields per group. Experiment reproduced 2 times. Scale bars in (B,D) – 200 µm.