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. 2017 Aug 16;7:8456. doi: 10.1038/s41598-017-08774-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FtsH is critical for in vivo survival of S. aureus during infection. (A) The role of FtsH in staphylococcal virulence during murine infection. The test strains were grown in TSB until exponential growth phase; then test strains were administered into eight mice via retro-orbital route. The significance of the survival was analyzed by Log-rank (Mantel-Cox) test. **p ≤ 0.01.The experiment was repeated, and similar results were observed. (B) The role of FtsH in the survival/growth of S. aureus during murine infection. At day 4 post infection, eight mice were killed, and the kidneys were harvested and ground. The colony forming unit (CFU) of S. aureus in the ground kidneys was measured by serial dilution method on tryptic soy agar. The detection limit is indicated by a dashed line.***p ≤ 0.001 by two-tailed Student’s t-test.