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. 2017 Aug 16;7:8306. doi: 10.1038/s41598-017-08859-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulated vehicle exhaust exposure (SVEE) model apparatus. SVEE consists of a whole body exposure system with two chambers fitted with tubing systems to allow inflow and outflow of gaseous mixtures. There is a cylinder that contains simulated mixture of vehicle exhaust. Gaseous mixture from the cylinder is blended with air in the mixer and then flow of the mixture is controlled using a flow regulator. Rats were subjected to SVEE in this apparatus.