Table 1.

Critical appraisal

	References	Study design	Patient selection	Threshold	Blinded index test results	Valid reference test	Disease progression	Verification	Withdrawal	Risk of bias	Representative patient sample	Extractable data	Applicability
I-FABP	Block et al. [37]	Cohort	●	○	●	●	●	○	●	Low	●	◐	Moderate
	Cronk et al. [40]	Cohort	●	◐	●	●	●	◐	●	Low	●	●	High
	Güzel et al. [41]	Case–control	◐	●	●	●	○	●	●	Moderate	●	●	High
	Kanda et al. [15]a	Case–control	◐	●	●	●	○	○	●	Moderate	●	●	High
	Kanda et al. [42]	Cohort	●	●	●	●	○	◐	●	Low	●	●	High
	Kittaka et al. [43]	Cohort	●	●	●	●	●	◐	●	Low	●	●	High
	Matsumoto et al. [44]	Cohort	●	●	●	●	●	◐	●	Low	●	●	High
	Matsumoto et al. [45]	Cohort	●	●	●	●	○	●	●	Low	●	●	High
	Shi et al. [38]	Cohort	●	●	●	●	●	◐	●	Low	●	●	High
	Thuijls et al. [46]	Cohort	●	●	●	●	●	◐	●	Low	●	●	High
	Uzun et al. [47]	Case–control	◐	●	●	◐	○	○	●	High	●	●	High
	Vermeulen et al. [48]	Cohort	●	●	●	●	●	◐	●	Low	●	●	High
	Voort et al. [39]a	Cohort	●	○	●	●	●	◐	●	Low	●	◐	Moderate
	Camkiran et al. [49]	Cohort	●	○	●	○	●	○	●	High	●	○	Low
	Lieberman et al. [50]a	Case–control	◐	○	●	○	○	○	●	High	◐	◐	Low
d-Lactate	Assadian et al. [51]	Cohort	●	○	●	●	●	●	●	Low	●	◐	High
	Block et al. [37]	Cohort	●	◐	●	●	●	◐	●	Low	●	●	High
	Collange et al. [52]	Cohort	●	◐	●	●	●	●	●	Low	●	○	Low
	Murray et al. [53]	Case–control	◐	◐	○	●	○	●	●	Moderate	●	●	High
	Poeze et al. [21]	Case–control	◐	◐	●	◐	○	◐	●	High	●	●	High
	Shi et al. [38]	Cohort	●	●	●	●	●	●	●	Low	●	●	High
	Voort et al. [39]a	Cohort	●	○	●	●	●	◐	●	Low	●	◐	Moderate
α-GST	Block et al. [37]	Cohort	●	◐	●	●	●	◐	●	Low	●	●	High
	Delaney et al. [54]	Case–control	◐	●	●	●	●	●	●	Low	●	●	High
	Gearhart et al. [55]	Cohort	●	◐	●	●	●	◐	◐	Low	●	●	High
IMA	Gunduz et al. [56]	Case–control	◐	●	●	●	●	●	●	Low	●	●	High
	Polk et al. [57]	Cohort	●	●	●	●	●	●	●	Low	●	●	High
C	Kulu et al. [58]	Case–control	○	●	●	●	○	●	●	Low	●	●	High

Patient selection: ● consecutive order, well described in- and exclusion criteria ◐ case–control with consecutive case selection ○ inappropriate exclusions. Threshold: ● based on ROC-analysis ◐ pre-specified ○ not reported. |Blinded index test results: ● yes ○ no/not reported. Valid reference standard: ● surgery, endoscopy, autopsy, full clinical recovery ◐ CT scanning, lab findings ○ none/not reported. Disease progression: ● < 12 h ○ ≥ 12 h/not reported. Verification: ●all patients received both index and reference test. Reference test was the same for all patients ◐ selected patients received equal reference tests ○ selected patients received different reference tests. Withdrawal: ● no loss to follow up ◐loss to follow up, reasons given ○ loss to follow up without reasons given/not reported. Representative patient sample: ● patients with suspected AMI ◐ healthy control group ○ non-matching domain. Extractable data: ● 2 × 2 table data extractable ◐ levels of biomarkers reported, no 2 × 2 data extractable ○ only correlation, no data on AMI

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