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. 2017 Aug 16;7:8480. doi: 10.1038/s41598-017-09095-3

Figure 4.

Figure 4

miR-181a mediated PRKAA1 expression by directly binding to its 3′-UTR in HEK293T and HT-22 cell lines. (a) Complementary sequences between wild-type (WT) or mutant PRKAA1(Mut) 3′-UTRs in the mRNA and miR-181a. (b) HEK293T cells or (c) HT-22 cells were co-transfected with the miR-181a agomir or miR-181a agomir control and the luciferase reporter construct containing the WT or Mut PRKAA1 3′-UTR. For each experiment, the results were normalized to the luciferase activity detected in the cells transfected with the miR-181a agomir control and the WT PRKAA1 3′-UTR. **p < 0.01, compared with the WT + miR-181a agomir control group. n = 5 per group. All values are presented as means ± SEM.