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. 2017 Aug 16;7:8480. doi: 10.1038/s41598-017-09095-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-181a was involved in LTM formation following CFC and OLT by regulating the expression and activity of PRKAA1. (a) Representative cropped western blots of PRKAA1, TSC2, p-TSC2, ACC, p-ACC, p70S6K and p-p70S6K protein levels in the mouse DH 2 h after CFC training. Original uncropped western blots are provided in a Supplementary Figure 1. (b,e) Statistical analysis of the western blot results. *p < 0.05, **p < 0.01, compared with the naïve group. ^## p < 0.01, compared with the CFC + antagomir control group. (f) Representative cropped western blots of PRKAA1, TSC2, p-TSC2, ACC, p-ACC, p70S6K and p-p70S6K protein levels in the mouse DH 2 h after OLT training. Original uncropped western blots are provided in a Supplementary Figure 2. (b,e) Statistical analysis of the western blot results. *p < 0.05, **p < 0.01, compared with the naïve group. ^## p < 0.01, compared with the OLT + antagomir control group. n = 5 per group. All values are presented as means ± SEM.