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. 2017 Aug 16;7:8321. doi: 10.1038/s41598-017-08304-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bleb-like microprojections from podocytes in CD2AP deficient mice. HIM images of podocyte cell body in WT (A), 3 week-old Cd2ap-KO (B), and 6 week-old Cd2ap-KO mice (C). The image of WT podocytes (A) shows a smooth podocyte cell body and well-organized interdigitating foot processes. Images of 3 and 6 week-old Cd2ap-KO podocytes (B,C) reveal numerous “bleb-shaped” microprojections with a diameter of 200–700 nm on the surface of the podocyte cell body. Most of these blebs have a narrow “neck” connected to the cell body. Scale bars, 500 μm in upper panels. (D) TEM images of 6 week-old Cd2ap-KO glomeruli show numerous vesicle-like structures. Some of these structures have a narrow “neck” connected to the podocyte cell body. Scale bar, 500 nm. Podo, podocyte; Endo, endocapillary space.