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. 2017 Aug 16;7:8325. doi: 10.1038/s41598-017-08449-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of cell membrane injury in E. coli O157:H7 treated by UV-A light with select compounds. (a) Permeability of E. coli O157:H7 to propidium iodide (PI) after treatment with GA (15 mM), GA (15 mM) + EDTA (1 mM), PG (10 mM), HCl (pH 3.1) or water for 30 min with or without UV-A exposure. Mean ± SD. Absolute fluorescence values were corrected by subtracting the fluorescence values for samples incubated in water and in dark. *Indicates that corrected fluorescence value was zero. (b) SEM images showing morphology of E. coli O157:H7 treated for 30 min by (A1-A3) water, (B1-B3) water + UV-A light, (C1-C3) 15 mM GA, (D1-D3) 15 mM GA + UV-A light, (E1-E3) 10 mM PG, (F1-F3) 10 mM PG + UV-A light.