Table 2. O-AcTase specific activities in wild-type and neuO mutant E. coli extracts.
| Relevant properties
|
NeuO activity, units
|
||||
|---|---|---|---|---|---|
| Strain (plasmid) | CUS-3 | neuO | K1 | Without CA | With CA |
| BW30270 | - | - | - | 0.1 | 0.1 |
| EV36 | - | - | + | <0.1 | <0.1 |
| EV291 | + | + | + | 3.2 | 7.5 |
| RS189 | + | + | + | ND | 5.5 |
| RS354 | - | - | + | <0.1 | <0.1 |
| EV708 | + | - | + | 0.1 | 0.1 |
| EV709 | + | - | + | ND | 0.1 |
| EV710 | + | - | + | ND | 0.1 |
| 10811 | + | + | + | 18.9 | 36.4 |
| EV711 | + | + | (+)* | 7.8 | 12.0 |
| EV712 | + | + | (+) | 8.7 | 11.2 |
| EV713 | + | + | - | <0.1 | 7.6 |
| EV708 (pSX785) | + | - | + | ND | 32.5 |
| EV708 (pSX786) | + | - | + | ND | <0.1 |
| DH5α (pSX785) | - | - | - | <0.1 | 48.5 |
| EV36 (pSX788) | - | - | + | ND | <0.1 |
ND, not determined.
*
Parentheses indicate the production of intracellular K1 antigen.